아라키돈산 유도 염증모델에서 LED 광조사에 의한 유전자 발현에 관한 연구
To investigate the differential expression of genes by 635nm LEDs irradiation in arachidonic acid-treated human gingivalfibroblasts, cDNA microarray was carried out. Human gingival fibroblasts were primary cultured and arachidonicacid was treated to induce inflammation. 635nm of wave length was used for LEDs irradiation. The experimental groupwas categorized into four group ; control, only LEDs irradiation group, only arachidonic acid-treated group and arachidonicacid-treated with LEDs irradiation group. The expression of 8,078 genes were increased and the expression of7,103 genes were decreased in only LEDs irradiation group. For arachidonic acid-treated with LEDs irradiation group,the expression of 6,815 genes were increased, while the expression of 8,031 genes were decreased comparing with onlyarachidonic acid-treated group. IL-13alpha2 receptor was the most expressed gene in LEDs irradiation group comparingwith control, followed by MMP3. Genes which the most down regulated was BIRC3 in LEDs irradiation group. PLABgenes was the most up-regulated in arachidonic acid treated with LEDs irradation group, followed by ranked RARRES1.Considering the classification by cell function, genes associated with signal transduction were the most affected by LEDsirradiation, followed by the genes associated with nucleoside, nucleotide and nucleic acid metabolism. In arachidonic acidtreated with LEDs irradiation, genes associated with signal transduction and protein metabolism were affected.Taken together, LEDs irradiation could affect various biological process and could identify many genes related to LEDsirradiation, which could be used for clinical application.