타액선종양에서 Survivin 발현에 관한 면역병리학적 연구
The purpose of this study was to evaluate the role of survivin in various salivary gland tumors. For this study, total18 cases of salivary gland tumors; 6 cases of benign and 12 cases of malignant tumors were used as experimentalgroup. In benign tumors; pleomorphic adenoma, oncocytoma, and in malignant tumors; adenoid cystic carcinoma, mucoepidermoidtumor, high grade and low grade malignancy each, adenocarcinoma, acinic cell adenocarcinoma cases wereincluded. And for the control group, fresh submandibular glands were attained from gnathosurgical specimen. All thespecimens, experimental, control group were fixed in 10% neutral formalin solutions, embedded in paraffin, sectioned5um or more in thickness, stained with the hematoxylin and eosin, mounted and examined under the microscope. Forthe immunohistochemical studies, all the specimens were activated with survivin monoclonal, and secondary antibodiesas usual manners, and taken photos on various pathologic fields analysed with the image analysis system, and evaluatedthe positive and negative stained area in the tumors on each images and statistically analyzed with SPSS 15.0program. Attained result as follows. In control group, in part, acini cells show positive reaction on the nuclei, negativeon the all most of the cytoplasm, more intense reaction on the cytoplasm and nuclei on the serous demilune(47.33%). In experimental group, all the specimens show survivin positive reaction on the cytoplasm with/or withoutpositive reaction on nuclei according to the tumors, in benign tumors; pleomorphic adenoma (63.48%), oncocytoma(56.31%), each and in malignant tumors; adenoid cystic carcinoma (87.6%), acinic cell adenocarcinoma (56.35%). adenocarcinoma(67.47%), mucoepidermoid carcinoma, low grade (70.76%). high grade (55.23%). Survivin expression showshigher in tumors compare to that on the control group (p<0.05), but between the malignant tumors no significant arenot noted(p>0.005). Survivin expression is strongly related to the malignancy of salivary gland tumors.