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31 2016 40권 6호

두 개의 분리된 구개치근을 가진 상악 제 3대구치의 근관치료

저자 최유리나

초록

The presence of the microorganisms of untreated canals is one of the main reasons of the failure in the endodontic treatment. The knowledge of variations in the canal systems of the tooth is important for the successful endodontic treatment. In the maxillary molars, the presence of the two separate palatal roots is very rare variations. Although there have been several case reports of maxillary first and second molars, the case reports of maxillary third molars are very few. This case report presents the endodontic treatment of a maxillary third molar with two separate palatal roots. It is important to notice the clinical signs and analyze the radiographs carefully. The use of a microscope is helpful for the visualization of pulpal chamber, and pulpal chamber floor should be investigated thoroughly with endodontic explorer. The straight-line access for all the canal orifices is important for the success in the endodontic treatment.

30 2016 40권 6호

측두하악관절에 발생된 양측성 활액낭의 증례

저자 유지용, 최은주, 이병도, 윤정훈, 이완

초록

Synovial cysts are found mainly in periarticular areas of the wrist, ankle, and knee, but also rarely in the temporomandibular joint (TMJ). Only 1 case of bilateral synovial cysts in the region of the TMJ has been reported. This case report described bilateral cysts in the TMJ in a 54-year-old Korean woman. T2-weighted magnetic resonance imaging (MRI) revealed bilateral oval cystic lesions lateral to the condylar capsule, whereas computed tomography (CT) apparently did not show the right hand side lesion. The cyst on the left side was surgically excised, and fine needle aspiration was performed on the right hand cyst. After 24 months, the long term follow-up showed no sign of recurrence.

29 2016 40권 6호

RAW 264.7 세포에서 투석시킨 커피 추출액의 세포생물학적 예비연구

저자 윤철수, 이석근

초록

In order to perform the biological investigation of coffee extract containing different molecules, it would be necessary to develop in vitro experimental system rather than animal experiment. Although the animal experiment treated via oral intake or intravenous injection may disclose the whole systemic effect, the in vitro cell culture experiment would be more convenient to analyze direct cellular effect of coffee extract than animal experiment. Therefore, this study was aimed to develop a dialysis method for the crude coffee extract to perform the biological investigation using murine macrophage cell line, RAW 264.7. First of all, the RAW 264.7 cells treated with dialyzed coffee extract were observed, and subsequently their protein extracts were analyzed by gel filtration chromatography, thin layer chromatography, and immunoprecipitation high performance liquid chromatography (IP-HPLC). Resultantly, it was found that the low dose (20μg/mL) of dialyzed coffee extract, about 5 cups of ordinary coffee drinking for human adult, enhanced the growth of RAW 264.7 cells by increased expression of β-actin and Ki-67, and also induced the anti-inflammatory effect by decreased expression of NFkB, TNFα, and LC3 contrast to the high dose (40μg/mL) of dialyzed coffee extract. The low dose of dialyzed coffee extract produced almost no harmful effect on RAW cell culture for 12 hours, rather than it produced stimulatory effect on RAW cells by increasing the cell number and enhancing the protein expression of β-actin, Ki-67. Therefore, it was thought that the low dose of dialyzed coffee extract is applicable to cell culture experiment without difficult purification procedures of coffee elements. In addition, as the contrast cellular effect between the low and high dose of coffee extract was found in this study, it was also presumed that the low dose of coffee extract may play an important role in the inflammatory reaction of murine macrophages.

28 2016 40권 6호

Kochia scoparia 열매 추출물이 구강 편평세포암종세포에서 예정된 괴사 유도

저자 한혜련, 박봉수, 김준형, 정승화, 김지연, 정성희, 김규천, 황대석, 김욱규, 김형우, 류미현

초록

The fruit of Kochia scoparia Scharder is traditionally used as a medicinal ingredient to treat allergic skin diseases and inflammatory diseases in China, Japan and Korea. Recently, several studies reported that K. scoparia had potential for the cytotoxicity of human cancer cells. To investigate the anti-cancer effect of K. scoparia on oral cancer and to determine the specific type of cell death induced by MEKS treatment. We investigated the anti-cancer effects of K. scoparia, methanol extract (MEKS) in HSC4 human oral cancer cells. We examined the effects of MEKS on the proliferation rate, cell cycle arrest, 7-AAD-ANNEXIN V double stain, reactive oxygen species (ROS) generation and activation of apoptosis and necroptosis-associated proteins in HSC4 cells. MTT assay results demonstrated that MEKS decreased the proliferation rates of HSC4 cells in a dose-dependent manner with an IC50 value of 45.3 μg/ml. MEKS at 50 μg/ml significantly increased the sub-G1 DNA contents of HSC4 cells to 84.8%, versus untreated cells. However, the activation of apoptosis-associated proteins such as cleaved caspase 3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase (PARP) did not detect. The level of Bax protein markedly increased in MEKS-treated HSC4 cells. In addition, the cell viability of the DPQ pre-treated HSC4 cells with MEKS treatment was significantly greater than that of MEKS treated-cells. These results suggest that MEKS inhibits cell proliferation and induces necroptosis in oral cancer cells and that MEKS may have potential chemotherapeutic value for the treatment of human oral cancer.

27 2016 40권 6호

젤여과 고수행 액체크로마토그라피 분석으로 확인된 침단백질복합체들의 항균 및 보호단백질 분포

저자 김연숙, 이석근

초록

The native human saliva obtained through the centrifugation of whole saliva showed characteristic salivary protein complex (SPC) peaks in gel filtration high performance liquid chromatography (HPLC) using Superose 12 column1,2). In the previous study the SPC peaks in chromatography were explored to know their composition and functions by different detection methods, but still the nature of SPCs was not clearly elucidated so far. In this study the SPC peaks were examined by direct antibody interaction in order to target different antimicrobial and protective proteins distributed in the SPCs via gel filtration HPLC. As the SPC peak shape and migration speed can be changed by antibody binding to specific proteins of SPC, it was found that mucin1 is evenly distribution in all SPCs, while PRPs are more abundant in the late dominant SPC than the early dominant SPC and also in the intermediated SPCs. Most of antimicrobial proteins including lysozyme, LL-37, lactoferrin, β-defensin-1, -2, -3, IgA, mucocidin, and α1-antitrypsin were more abundant in the late dominant SPC than the early dominant SPC, while histatin showed relatively even distribution in all SPCs. Therefore, it was presumed that the late dominant SPC containing abundant antimicrobial and protective proteins could be applied as a biomarker to measure the defensive potential of whole saliva in oral diseases.

26 2016 40권 6호

수은과 Nitrosamine이 인체상피세포의 발암화에 미치는 영향

저자 김문수, 김진수, 최소영

초록

NNK (4-(methylnitrosamino)―1-(3-pyridyl)-1-butanone) is a major form of nitrosamine abundant in cigarette smoke and is a powerful carcinogen. Mercury is a major component of the amalgam that is widely used as dental filling material. Concurrent exposure to these two agents may result in their interaction and alter their carcinogenic potential. The present study used an immortalized human epithelial cell system that allows continuous exposure to potential carcinogens, in an attempt to elaborate the carcinogenic potential of mercury and NNK in humans. Cytotoxicity of mercury chloride and NNK was measured by an MTT assay. Parameters of neoplastic cellular transformation such as cell saturation density, soft-agar colony formation, and cell aggregation were analyzed to examine the carcinogenic potential of mercury chloride and NNK. The study showed that exposure to mercury chloride with NNK resulted in increased soft agar colony formation and cell aggregation. ROS generation by mercury chloride was further enhanced by treatment with NNK. The apoptosis that was observed following mercury chloride exposure was further increased upon co-treatment with NNK. The interaction between these two agents was also observed in cytokine mRNA induction. In the present study, mercury alone did not seem to pose a significant threat as a carcinogen, but it may have potential to enhance the carcinogenic potential of a known carcinogen from cigarette smoke. The present study provides valuable data regarding the evaluation of potential carcinogenic risk of mercury chloride and NNK on concurrent exposure.

25 2016 40권 5호

특발성 치근외흡수의 처치: 증례 보고

저자 최유리나, 김현하, 박수정

초록

External root resorption is one of the rare pathological condition of tooth. It is occurred when periodontal ligament or cementum on the root surface are either damaged or denuded. The causes and classifications have been variously described. Because of the various causes and patterns, and absence of sufficient and consistent evidence for treatment, proper diagnosis and treatment plan based on clinician’s experience and close communication with patient is important. This case report represents the rare case of external root resorption and the treatment. The tooth was retained functionally in oral cavity showing good healing state in 2-year follow-up.

24 2016 40권 5호

복잡치아종을 보이는 석회화낭종성 치성종양 증례- 유령세포에서 베타-카테닌, GADD45, 및 LC3의 면역조직화학적 과발현 -

저자 이상신, 김연숙, 이석근

초록

A nineteen years old male patient showed a cystic lesion in left maxillary canine to premolar area (#23-#25). This lesion was asymptomatic, and found during his routine radiological check in local clinic. In the radiological observation the cystic lesion showed round radiolucent image containing many calcified bodies which were usually small but irregular in shape, expanding tumorously and resulted in the displacement of canine and second premolar in the absence of first premolar. The lesion was surgically enucleated, and a cystic fibrous tissue containing abnormal teeth was removed and examined pathologically. With the histological observation of tumorous odontogenic epithelium including many ghost cells, which were closely associated with abortive teeth, the lesion was finally diagnosed as CCOT associated with complex odontoma. The ghost cells of CCOT was strongly positive for β-catenin, GADD45, and LC3, and slightly positive for MMP-9, while they were rarely positive for BCL2, Wnt1, HSP-70, and p38. Therefore, it was presumed that the ghost cells of CCOT might undergo dormant cell state through altered cytodifferentiation stimulated by severe growth arrest, DNA damage signaling, and abundant autophage formation.

23 2016 40권 5호

구개부에 발생한 구강 섬유림프종- 증례보고-

저자 박정원, 정태영, 박상준

초록

Oral Fibrolipoma on Palate: A case report Jung-Won Park, Tea-Young Jung, Sang-Jun Park Department of Oral and Maxillofacial Surgery, Inje University Busan Paik Hospital, Busan, Korea Fibrolipoma, a variant of lipoma, is rare in oral cavity, and the pathogenesis is uncertain. The diagnosis and differentiation of fibrolipoma with other clinically similar lesions is very essential for proper treatment planning. We present a case of fibrolipoma on palate of a 28-year-old male.

22 2016 40권 5호

혀에 발생한 선천 흑색증성 모반-증례보고-

저자 김강혁, 조정우, 정승환, 김진수, 박재억, 김창현

초록

Oral hyperpigmentation is common in patients older 40 years. But lesions in a newborn are unusual and congenital melanotic macule of the tongue has rarely been reported. A 2-month-old infant with 3 pigmented lesion on the right side of the dorsal tongue was evaluated. They were brown black but not homogeneous in color, smooth, nonblanchable, and nonpalpable, with irregular margins. We recommend excisional biopsy under general anesthesia because of possibility of malignancy, but parents refuse invasive procedure. On a following-up examination of the child at the age of 1 year, the pigmented lesions were unchanged. We report a case of congenital melanotic macules on the tongue and a review of literature about the lesion.

21 2016 40권 5호

점액표피양암종세포주에서 Bax 활성화 유도를 통한 Cisplatin의 항암효능

저자 유현주, 조남표, 정요셉, 신지애

초록

Cisplatin is a well-known platinum-containing anti-cancer drug against bladder, ovarian, lung and testicular cancer. However, the potential effects and molecular targets of cisplatin in human mucoepidermoid carcinoma (MEC) are not fully understood. Here, we investigated the apoptotic effect and underlying mechanism of cisplatin in human MEC cells. The potential effects of cisplatin were evaluated by trypan blue exclusion assay, Western blotting, 4’-6-diamidino-2-phenylindole (DAPI) staining, live/dead assay and immunocytochemistry. Cisplatin suppressed cell growth and enhanced expression of cleaved PAPR in MC3 and YD15 cells. Cisplatin caused morphological change of nuclei and increased the number of ethidium homodimer-1-stained cells. In addition, cisplatin commonly increased Bax activation in both cells, while other Bcl-2 family proteins were not affected. These results suggest that cisplatin might induce apoptosis by activating Bax protein, which would provide baseline data for development of effective treatment strategy against MEC.

20 2016 40권 4호

상악골에서 골-섬유조직이 과오종 같은 증식을 보이는 동맥류뼈낭종

저자 이상신, 김연숙, 이석근

초록

Aneurysmal bone cyst (ABC) in maxilla is a rare and benign lesion but shows extensive bony destruction, occasionally accompanied with secondary osseous lesions, i.e., central giant cell granuloma, ossifying fibroma, fibrous dysplasia, etc. As the pathogenesis of ABC has not been clearly defined, ABC is diagnostically challenged due to its variable histological features. A 17-year-old boy showed a huge radiolucent lesion at right anterior maxilla, which was accidentally found in routine dental-radiological examination for orthodontic treatment. He had no medical history of systemic disease, and did not remember any traumatic experience on his right anterior maxilla. The radiolucent lesion involved periapical area from right central incisor to right first premolar, and was clinically diagnosed as odontogenic keratocyst. During surgical operation a cyst-like sac was enucleated with severe hemorrhage. In the histological observation the thick fibrous sac showed no lining epithelium, and its luminal side disclosed multiple aneurysmal spaces which were shrunken and almost obliterated. The fibrous sac itself was hyperplastic with abundant vascular channels, and produced fibromatous thickening associated with ossifying trabecular bones. This fibro-osseous tissue was hamartomatous, which was not directly connected and organized with marrow bone of maxilla. Finally, the present case was diagnosed as secondary type ABC differentially from traumatic bone cyst (TBC), odontogenic cyst, and central reparative granuloma. And it was presumed that the hamartomatous proliferation of fibro-osseous tissue in the cystic sac of ABC could produce the swelling pressure effect in the bone marrow similar to the overgrowth of central giant cell granuloma, ossifying fibroma, fibrous dysplasia, etc., in the secondary type ABC.

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