학회지

학회지 바로가기

43권 6호 허혈성 암세포에서 상피-간엽 이행과 연관된 유전자 발현 프로필

페이지 정보

profile_image
작성자 최고관리자
댓글 0건 조회 632회 작성일 20-04-10 14:13

본문

Hypoxia is one of the most common features of cancer. It is also associated with cancer progression and the acquisition of aggressiveness,
which includes invasion and metastasis. Oral squamous cell carcinoma accounts for 90% of all oral cancers, and its 5-year survival rate
is about 50%. Despite various attempts and trials, its prognosis has not improved. Among numerous adverse prognostic factors, hypoxia
is suspected as one of the most important factors, as it increases the aggressiveness of oral cancer cells. We attempted to observe
the effect of hypoxia on the expression of epithelial-mesenchymal transition markers in oral cancer cells. We analyzed and compared both
the mRNA and protein expression levels of epithelial-mesenchymal markers using qRT-PCR and western blotting in both normoxic and
hypoxic YD10B oral squamous cell carcinoma cells. Eighty-six genes were analyzed through real-time PCR using commercial microarray
plates, performed in triplicate. Among the 86 genes, the expression of 24 were increased (≥ 2 fold) by hypoxia, while that of three genes
was decreased (≥ 2 fold). Hypoxia significantly affects epithelial-mesenchymal transition-related genes. Further studies on the regulation
of these genes may help to develop more efficient therapeutic modalities for oral cancer and to improve prognosis of oral cancer patients.
Key words : Hypoxia, Oral cancer, Epithelial-mesenchymal transition, TCF-4

첨부파일

댓글목록

등록된 댓글이 없습니다.