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18 2008

Quinolone 내성을 나타내는 Salmonella enterica subsp. ente

저자 김은기, 오세아, 김연

초록

In order to investigate the resistance patterns and molecular characteristics of resistance gene of 9 NA-resistantSalmonella enterica subsp. enterica serovar Enteritidis, Total of 101 isolates were isolated from stool sampes from 2005to 2006. Among them, 48 isolates(47.5%) were identified S. Enteritidis, 24 isolates(23.8%) S. Typhi, 8 isolates(42.5%) S.Typhimurium, 7 isolates(6.9%) S. Paratyphi A and 14 isolates (13.9%) others Salmonellas. All of S. Enteritidis were resistantto ampicillin(43.8%), ticarcillin(43.8%), streptomycin(37.5%), chloramphenicol(29.2%), tetracycline(10.4%) and nalidixicacid(18.8%), respectively. All nalidixic acid-resistance isolates represented one point mutation in the quinolone resistancedetermining region(QRDR) of gyrA gene. Among them, 8(89%) isolates were substituted Tyr for Ser at positionamino acid 83th or 1(11%) isolate was substituted Asn for Asp at position amino acid 87th. In consequence, Continuedsurveillance of NA-resistance among non-Typhi S. entetica isolates is needed to mitigate the increasing prevalence ofnalidixic acid resistance.

17 2008

골대체재인 합성 수산화인회석과 Anorganic Bovine Bone Mineral의 생체

저자 이석준

초록

It is necessary to improve the esthetic and function in the patient with oral and maxillofacial bone defects. Syntheticbone substitute materials and anorganic bovine bone mineral(ABBM) have been used for clinical restoration.The purpose of this study was to observe the biocompatibility and bone formation of synthetic hydroxyapatite(SHA)and ABBM in hole of rabbit's tibia. After specimens with SHA and ABBM at 8 weeks were fixed in 10% neutral formalinsolution, dehydrated, and embedded with spurr low viscosity, they were cut by 500um with slow diamond wheel saw andgrinded up to 200um in thickness. These specimens were coated with carbon and examined with r efraction microscopefor bone density. Refraction microscopic features of 8 weeks in synthetic HA showed network-like new bone formingtrabecular pattern attached to resorbed HA. Less well calcified trabecular bone surrounding conglomerated HAs showedirregular arrangement of numerous osteocytes. There was not completely filled in defected area by new bone trabecular.New trabecular bone formation by ABBM was more prominent and completely compacted in defect hole at 8 weeks. Itsuggested that although bone formation activity of AMMB might be superior to that on synthetic HA, both group wouldbe the good biocompatibility in this experiment.

16 2008

아가로즈-셀룰로오스 하이브리드 신소재의 물리화학적 특성과 의학및 생활 보건의 적용

저자 임재길, 윤정로, 김성

초록

In order to develop a protective carrier scaffolder for the external usage of medical and hygienic materials, three essentialprotective elements existing in nature, i.e., algin, cellulose, and calcium phosphate apatite, were investigated.The algin is a main skeletal component of sea weeds, the cellulose is of vegetables, and the calcium phosphate apatite isof vertebral animals. In the present study we select the agarose which is a derivative from algin, the cellulose fiber obtainedfrom skin of sea squirt, calcium oxide purified from shell powder, and tricalcium phosphate apatite purchasedcommercially. Consequently, the agarose-cellulose hybrid was made by the hydrogen bonds intermediating the calciumphosphate apatite between agarose and cellulose molecules. As the calcium phosphate apatite is formed by the additionof calcium hydroxide into tricalcium phosphate solution, we used calcium oxide to accelerate the hybridization betweenthe agarose and calcium phosphate apatite and also between the cellulose and calcium phosphate apatite. In the phasecontrast microscopic observation the agarose-cellulose hybrid showed more compact matrix structure than the mixture ofagarose and cellulose. The agarose-cellulose hybrid showed increased storage modulus but decreased loss modulus inRheometer test compared to those of the other materials tested in this study, representing that the agarose-cellulosehybrid has the highest elasticity among them and similar water capacity to agarose. The agarose-cellulose hybrid showedthe strongest antimicrobial effect in bacteria killing assay than the other materials, and also it showed a potent bloodclotting effect but no immunological hypersensitivity on the human skin. From the above results we presumed that thenobel material, agarose-cellulose hybrid, is a compact scaffolding matrix which has proper elasticity, high capacity tohold substrates, and antimicrobial and blood clotting property potent enough to carry the bio-medical and hygienic materialsfor external treatment safely.

15 2008

SGT 세포주에 미치는 Taxol의 Apoptosis 효과

저자 이종헌

초록

Taxol(paclitaxel) is used in chemotherapy against several cancer. Treatment of tumor cell lines with taxol inducesapoptosis, but exact mechanism is not sufficiently understood. The purpose of this study was to investigate apoptosis bythe inhibitory effect of paclitaxel on the motility properties of human salivary gland adenocarcinoma cell lines. Paclitaxelinhibited cell motility induced by soluble and immobilized attractant. It suggested that paclitaxel would be a potent inhibitorof salivary gland adenocarcinoma cell motility independent of its cytotoxic and apoptotic activity.

14 2008

NFI-C 결손이 생쥐 치수 세포의 유전자 발현에 미치는 영향

저자 이동설, 김성일, 한평

초록

Nuclear factor I-C (NFI-C) null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation,and thus molar lacking roots. However, the mechanism by which the disruption of NFI-C gene affect the expression ofother genes in dental pulp cells remains unknown. In this study, in order to understand this mechanism, the gene expressionof pulp cells in NFI-C deficient mice were compared to those of wild-type mice by cDNA microarray analysis.According to the cDNA microarray profile comparison, the disruption of NFI-C gene increased the expression of TGF-βand TGF-β receptor, whereas it decreased the expression of Smad proteins. Interestingly, most of the FGF-related geneswere down-regulated in pulp cells by NFI-C gene disruption. Among the cell cycle-related genes, the expression of p16and p18 were increased by NFI-C disruption, but the expression of cy clin E1 and cy clin D1 were decreased by NFI-Cdisruption. These results indicate that the disturbance of NFI-C gene suppressed the proliferation of pulp cells andup-regulated the expression of TGF-β and its downstream signaling molecules during root formation, contributing to theformation of short root containing abnormal dentin.

13 2008

사람 두경부 편평세포암종 FaDu 세포에서 BCH에 의한 세포성장 억제기전

저자 이상신, 김상율, 송지

초록

"Amino acid transporters are essential for the growth and proliferation in all living cells. Among the amino acid transporters,the system L amino acid transporters are the major nutrient transport system responsible for theNa+-independent transport of neutral amino acids including several essential amino acids. The L-type amino acid transporter1 (LAT1), an isoform of system L amino acid transporter, is highly expressed in cancer cells to support their continuousgrowth and proliferation. 2-Aminobicyclo-(2,2,1)-heptane-2-carboxylic acid(BCH) is a model compound for studyof amino acid transporter as a system L selective inhibitor. We have examined the effect and mechanism of BCH on cellgrowth suppression in FaDu human head and neck squamous cell carcinoma. The BCH inhibited the L-leucine transportin a concentration-dependent manner with a IC 50 value of 43.8±4.3μM. The majority of L-leucine uptake is, therefore,mediated by LAT1 in FaDu cells. The growth of FaDu cells was inhibited by BCH in the time- and concentration-dependent manners. The formation of DNA ladder was not observed with BCH treatment in the cells.Furthermore, the proteolytic processing of caspase-3 and caspase-7 in the cells was not detected by BCH treatment.These results suggest that the BCH inhibits the growth of FaDu human head and neck squamous cell carcinoma throughthe intracellular depletion of neutral amino acids for cell growth without apoptotic processing. "

12 2008

구개에 발생한 CD30 양성 림프증식 질환

저자 윤정훈, 김규식, 안상

초록

Traumatic eosinophilic granuloma(TEG) of the oral mucosa is considered to be a reactive benign condition. Histologyrevealed diffuse mixed infiltration of eosinophil and atypical mononuclear cells. We have described an additional case ofTEG simulating oral malignany where immunohistochemistry revealed the presence of CD30+ large atypical cells. TheCD30+ lymphoproliferative disorder(LPD) of oral mucosa, although rare, has also been described. In this case, there wasscattered distribution of CD30+ cells. After the incisional biopsy, the remainder of oral lesion got disappeared progressivelyand there is no sign of recurrence. We believe that this case could be a reactive rather than neoplastic process,and it has been suggested that a subset of TEG could be included within the spectrum of CD30+ LPDs. Therefore,oral surgeon and pathologists’ awareness of this condition will reduce the likelihood of misdiagnosis and inappropriateaggressive treatment for this benign, self-limiting lesion.

11 2008

하악골에 발생한 콜레스테롤 육아종

저자 김지홍, 홍성두, 이재

초록

Cholesterol granuloma(CG) occurs frequently in association with chronic middle ear diseases, particularly diseases inthe mastoid antrum and air cells of the temporal bone, and much less frequently in paranasal sinuses. It occurs frequentlysecondary to massive hemorrhage of oral and paraoral cysts. However, It has never been reported to occur solelywithout any association with preexisting lesion in the mandible. We experienced development of unusual cholesterolgranuloma in the mandible. Seventy year old female presented diffuse hard swelling on the left mandibular area withlymphadenopathy of the left cervical lymph node. Radiographic examination showed a well circumscribed multilocular radiolucencyresembling soap bubble appearance with tooth displacement and root resorption, leading to the radiogrphicimpression of dentigerous cyst or odontogenic cyst or ameloblastoma. CT showed bucco-lingually undulating expansilelesion with corticated margin from the left posterior mandibular body to the anterior ramus, including #46, #47 and #48,and the mass showed slightly lower attenuation than muscle. leading to the impression of ameloblastoma. The mass aftersurgical excision composed of 2 sac like structures, measuring 4.0cm, and 2.7cm in diameter respectively. One sac wastightly attached to the #46, resembling dentigerous cyst.Microscopic examination showed a large number of cholesterol clefts in association with hemorrhage, hemosiderin pigmentsand foreign body giant cells. There was no evidence of cyst or other lesions.

10 2008

살모넬라의 세포질외부-기능 시그마 인자δE 발현에 미치는 철이온 농도의 영향

저자 방일수

초록

Biological organisms require iron for optimal metabolism. Intracellular pathogens also must secure iron especially duringinfection of animal hosts expressing NRAMP(natural resistance-associated macrophage protein), a transporter proteinsequestering metal ions from pathogens. This study shows that extracytoplasmic function sigma factor σE is required forSalmonella virulence in NRAMP1-expressing mice, and further shows that iron deprivation turns on σE expression ofSalmonella. The virulence of σE -deficient Salmonella is completely attenuated in C3H/HeN mice while wild typeSalmonella kills all mice. Addition of an iron-chelator DTPA(Diethylene triamine pentaacetic acid) to culture media inducesσEexpression of Salmonella, but iron supplementation abrogates this induction. These findings suggest that ironlimitation in host macrophages can trigger σE -dependent virulence system of Salmonella that may include bacterial ironhomeostasis.

9 2008

백혈병 세포주에서 Actin 세포골격 변화에 따른 산화적 스트레스에 의한 세포사멸 유도

저자 김지영, 박혜련, 안원

초록

In this study, the apoptotic effects of the actin disruption agent, latrunculin B(LB) have been investigated on p53 deficientchronic myeloid leukemia cell line K562. A dose-dependent decrease in K562 cell proliferation was observed afterLB treatment with maximum decrease in cell proliferation being at 1.5μM where the percent inhibition was 66.53%.F-actin stained with TRITC-phalloidin was shown as a peripheral ring or appeared diffusely distributed throughout thecytoplasm in untreated cells, this actin ring was decreased following LB treatment, and even large focal actin aggregateswere formed. Treatment of K562 with LB(1.5μM) generated ROS substantially. LB activated expression in a dose-dependentmanner. Therefore it can be concluded that LB, depolymerising agent of actin, induces apoptosis by producingROS and up-regulating NF-kB and COX-2 activation.

8 2008

치은섬유아세포 성장 조절을 위한 LED조사의 최적화조건 탐색

저자 류재록, 김인애, 정민

초록

The purpose of the present study is to investigate the optimal wavelength, frequency and energy density for set upthe photobiologic treatment of periodontal disease. To establish the present study,λscan of 500㎚to 900㎚ was used tosearch the optimal wavelength for maximal proliferation of human gingival fibroblasts. Cell proliferation assay was carriedout as MTT assay. Light intensity of 0.8 to 3.25mW, frequency of 0 to 584㎐and 0 to 2hours was applied for investigationof optimal energy density, frequency and applied duration. Finally, 628㎚ with 1mW/cm2 for 1hour of LED irradiationresulted in maximal proliferation of gingival fibroblasts. These results suggest that LED irradiation on gingivalfibroblast show different proliferation according to the condition of irradiation, and demonstrate that LED irradiationcan control the quantity of cell proliferation.

7 2008

최근 10년 동안 의뢰된 35례의 임플란트 주위염 조직 생검에서 보이는 병리학적 소견

저자 이상신, 김상율, 송지

초록

35 peri-implantitis recently referred for 10 years showed four types of inflammatory lesions, such as mild granulomatouslesion(n=5), severe granulomatous lesion(n=4), severe inflammatory fibrous scar tissue(n=15), severe abscessformation(n=11). However, the inflammatory lesions were usually localized at the peri-implant area accompanying compensatoryhyperplasia of fibrous connective tissue. The fibrous scar and the necrotic abscess frequently occurred dependon the severity of inflammatory reaction. Among 30 cases of severe inflammatory lesions, only 2 cases involved condensingosteitis in adjacent alveolar bone. Thus, we suppose that the inflammatory progression of peri-implantitis couldbe partly inhibited by the hyperplastic fibrous stromal tissue stimulated by implant material. And more, the focal abscessformed around the implant can be easily drainaged through the fibrous tract of implant pathway, resulted in thechronic persistent inflammatory granulomatous lesion, that is contrast to the common socket granuloma after toothextraction. However, depend on the degree of inflammatory reaction in the peri-implantitis the inflamed fibrous collagenoustissues, unregenerated graft materials, necrotic abscess and sequestra should be removed by surgical interventionand followed by antibiotic therapy, because the peri-implant tissue is as vivid as the normal periodontium for the inflammatorydefense system. Therefore, we suggest that the inflammatory lesions of peri-implantitis be carefully treatedto improve the prognosis for the following dental treatments.

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