면역침전 고수행 액체 크로마토그래피를 이용한 타액분석 방법의 개발
As the human mixed saliva plays important roles for the protection, regeneration, immunity, and molecular transfer/signaling in the oral and gastro-intestinal mucosa, the salivary contents have great implications for the generalhealth of human body. Nevertheless, the analysis method of human saliva has not been well developed up to date, becausethe proteins of mixed saliva are rapidly interacted with each other and easily degraded by proteolytic enzymesand microorganisms. This study aims to develop an immunoprecipitation-based high performance liquid chromatography(IP-HPLC) for the analysis of human mixed saliva. The representative IP-HPLC analyses were performed to compareamong different subjects in variable general conditions. Compared to the normal control the subjects suffered frombacterial infections of gastro-intestinal enteritis, chronic periodontitis, and acute necrotizing gingivo-stomatitisshowed dramatic increase of LL-37 level depending on the severity of diseases, while the subject suffered from Herpesstomatitis, a viral infection showed great increase of β-defensin 2. These data indicate that LL-37 in human mixedsaliva is more responsible to the bacterial infections of gastro-intestinal enteritis, chronic periodontitis, and acute necrotizinggingivo-stomatitis, while β-defensin 2 is more responsible to the viral infection of Herpes stomatitis. Thisstudy also suggeststhat the IP-HPLC be easily applicable to the wide range of biological samples for the quantitativeanalysis of an objective protein.