광주광역시에 거주하는 고령자의 타액 내 병원성 칸디다종의 동정에 관한 연구
Candida albicans and their associated Candida species are opportunistic pathogens which exists as normal flora inthe oral cavities of healthy individuals. In response to physiological changes in the host, these yeasts can becomepathogenic, resulting in oral candidiasis. The rapid detection and identification of Candida species in clinical laboratoriesare extremely important for the management of patients with hematogenous candidiasis. The presently availableculture and biochemical methods for detection and species identification of Candida are time-consuming and lackthe required sensitivity and specificity. In this study, we have established a seminested PCR (snPCR) using universaland species-specific primers for detection of Candida species in saliva. The universal outer primers amplified the 3endof 5.8S ribosomal DNA (rDNA) and the 5end of 28S rDNA, including the internally transcribed spacer 2 (ITS2), generating350- to 410-bp fragments from the four commonly encountered Candida spp., viz., C. albicans, C. tropicalis,C. glabrata, and C. parapsilosis. The saliva from 331 healthy and, over 50 years of aged people lived in Dong-gu,Gwangu city, was collected. Total DNA were extracted by Hoffman-Winston yeast total DNA prep. method and performedt he s nP CR. R esults appeared to b e negative on 292 people ( 88.2%), however, 2 6 people ( 7.9%) were p ositiveCandida albicans, 6 people (1.8%) were positive Candida glabrata, 5 people (1.5%) were positive Candida tropicalis, andonly 2 person (0.6%) were positive Candida parapsilosis. These result showed that detection and identification ofCandida species could be established by saliva analysis, so that snPCR on saliva is useful method of diagnosis of clinicalfields.