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Total 36건 3 페이지
12 2011

(-)-Epigallocatechin-3-gallate(EGCG)가 타액선 암세포의 제1형 교원질 부착 및 이동에 미치는 영향

저자 윤효은, 윤정훈, 안상건

초록

"EGCG has inhibitory effect on a variety of cancers by inducing apoptosis and cell cycle arrest or inhibiting angiogenesis and metastasis. EGCG has been found to induce apoptosis in salivary gland carcinoma cells. But the potential anti-invasive effect of EGCG in salivary gland cancer has not been studied yet. The aim of this study is to evaluate the effect of EGCG on salivary gland adenocarcinoma SGT cell adhesion and migration to Type I collagen treatment. Western blot, adhesion and migration assay were performed to evaluate the impacts of EGCG on the expression of MMP-2/-9 and its upstream signaling molecules after treatment of type I collagen. SGT cell adhesion to type I collagen is significantly suppressed by EGCG. EGCG decreased expression of β1 integrin, phosphorylation of FAK, MMP-2/-9 compared with type I collagen treatment. In addition, EGCG inhibited the migration of SGT cells treated with type I collagen. These results suggest that EGCG could effectively inhibit the invasion and migration of human SGT cells by downregulating the expression of β1 integrin and MMP-2/-9 and phosphorylation of FAK, Akt, and Erk. Adhesion and migration to type I collagen of SGT cells can be influenced through EGCG."

11 2011

경부에서 전이성종양을 닮은 가성육종성 결절근막염의 증례보고

저자 김민근, 송지영, 김성곤, 박영욱, 윤종상, 이석근, 이상신

초록

"The present case occurred in the cervical area of 51 years old male, who was generally healthy but recently complained of cervical swelling with mild pain. The tumor recently grew rapidly for 5 months. He was referred from local hospital in the diagnosis of metastatic tumor in cervical lymph. In the biopsy procedure, the tumor mass was ill-defined and not associated with cervical lymph node. The microsection showed a highly proliferative fibrous tissue infiltrative growth into the periphery. The spindle tumor cells were stained red in Masson trichrome stain, and strongly positive for PCNA and β-catenine, and partly positive for α-smooth muscle actin (α-SMA), but negative for S-100 and desmin. The differential diagnosis in primary biopsy examination was made as leiomyosarcoma, malignant schwannoma, and mesenchymal sarcoma. Under the diagnosis of sarcoma the patient was operated for tumor resection including cervical lymph nodes. The tumor mass was tightly attached on the lower border of left mandibular body. After the surgical operation the tumor tissue was more investigated with further immunohistochemical stainings, and discussed with several pathologists in Gangneung area. Finally the tumor was turned out to be a nodular fasciitis with pseudosarcomatous proliferation, and also confirmed that the pseudosarcomatous spindle cells belonged to the myofibroblasts originated from the fibrovascular fascial tissue. The present study demonstrated a rare case of nodular fasciitis, which should be differentially diagnosed from the malignant head and neck tumors."

10 2011

중심성 과립세포치성종양의 면역조직학적 특성

저자 권대근, 정재광, 박의균, 신홍인

초록

"The central granular cell odontogenic tumor is a rare benign odontogenic neoplasm of uncertain hisotogenesis characterized by varying amounts of eosinophilic granular cells and apparently inactive odontogenic epithelium with variable presence of calcified tissue. We present a case of central granular cell odontogenic tumor involving the maxilla of 35-year-old man with immunohistochemical characterization of granular cells. In microscopic view, the granular cells densely packed in sheets and lobules with abundant eosinophilic, granular cytoplasm and eccentric round-to-ovoid nuclei revealed immunoreactivity for vimentin, α1-antitrysin and CD68, and NSE but not for cytokeratin and S-100 protein while the interspersed odontogenic epithelial cells were positive for cytokeratin only. Granular cells also revealed strong PAS staining. Numerous concentric structured round to ovoid calcified aggregates were also noted. The lesion was treated with excision without recurrence for 8 years. Our immuohistochemical staining findings also suggest that the granular cells of central granular cell odontogenic tumor are mesenchymal in origin with possible histiocytic differentiation."

9 2011

하악골에 발생한 중심성 과립세포 치성종양

저자 윤혜정, 최소영, 홍성두, 홍삼표, 이재일

초록

"Central granular cell odontogenic tumor (CGCOT) is a rare benign odontogenic neoplasm, with approximately 30 cases having been reported. The pathogenesis of CGCOT as well as the designation of this lesion is controversial because of unknown histogenesis of the granular cell. We present an additional case of CGCOT involving the mandible of a 50-year-old Korean man who complained of asymptomatic swelling of the right buccal gingiva. Current lesion is microscopically characterized by densely packed polyhedral granular cells surrounding interspersed islands or strands of odontogenic epithelium. Immunohistochemically, granular cells were positive for Vimentin and CD68, and negative for cytokeratin and S-100. These features support a mesenchymal origin for the granular cells as other results previously reported."

8 2011

태권도선수의 시합 전 생리적 반응

저자 김형대, 오연풍, 이석준

초록

"Exercise training effciency depends on the training load, as well as on the athlete's ability to tolerate it. Taekwondo has been recognized as an Olympic sport in the 2000 Sydney Olympic games. Taekwondo competitions are regularly organized at the regional, national and international level according to the athlete's age and skill level. An integrated psychobiological stress-related measurement during a Taekwondo competition could be of considerable interest for studies on athlete performanc and behavior. The aim of this study was to evaluate the effects of an official Taekwondo competition on the heart rate(HR) and galvanic skin response(GSR) which represent stress score. each 5 male and female Taekwondo athletics were participated in this experiment. All persons were raised GSR and HR during competition and punching or kicking on opposite athletics. The results were statistically significant. It was suggested that all athleics had stress about competition."

7 2011

광주 거주자의 타액 검사와 혈액 검사의 비교 고찰

저자 조성훈, 김서연, 고영종, 임원봉, 오미라, 김지선, 김인애, 권혁일, Sandeep Karna, 김옥준, 최홍란

초록

"A blood test is a laboratory analysis performed on a blood sample that is usually extracted from a vein in the arm using a needle, or via fingerprick. They are used to determine physiological and biochemical states, such as disease, mineral contents, drug effectiveness, and organ function. Although the term blood test is used, most routine tests (except for most haematology) are done on plasma or serum, instead of blood cells. Main advantage of using saliva in diagnostics is easy and non invasive sample taking compared to peripheral blood. According to the study published, saliva contains more than 20 percent of the proteins found in blood. The purpose of present study is to compare biochemical enzymes in saliva and in blood serum and to evaluate the usefulness of saliva specimens instead of blood in dental clinic. The saliva from 215 healthy over 50 years of aged people lived in Dong-gu district, Gwangu city was collected and the analysis was performed by six enzyme-linked immunosorbent assays (ELISA). ELISA results were compared with blood chemistry results. The values or patterns on Alanine Aminotransperase (ALT), Aspartate Aminotransperase (AST), Cholesterol and Triglyceride in saliva were not correlated with those in blood serum. However, Albumine and γ-glutamyl transpeptidase (γ-GTP) were followed the positive relationship with blood chemistry. These result showed that detection and identification of Albumine and γ-GTP level could be established by saliva ELISA analysis, so that ELISA assay on saliva could be useful alternative to serum testing."

6 2011

티타늄 Particle농도에 따른 치주인대세포내 세포독성 및 OPG, RANKL의 발현에 관한 연구

저자 조형준, 나유진, 정용석, 홍지숙, 김윤지, 이종헌, 천재식, 박양호

초록

"The purpose of this study was to investigate the effect of cytokine-induced osteoclastogenesis on tooth movement related to orthodontic force. We evaluated the cytotoxicity as well as the expression of OPG and RANKL, which influence the homeostasis of bone metabolism. Titanium particles were applied to human periodontal ligament cells and subcultured fourth generation cells. The ALP assay and the MTT assay were used to assess changes in cytotoxicity. After 48 hours, cytotoxicity increased proportionally with the concentration of titanium. With 20 mg, the cytotoxicity was the lowest. RT-PCR was used for assessing mRNA levels of OPG and RANKL; after 96 hours, the mRNAs of OPG and RANKL increased steeply. A western blot analysis showed that with 20 mg of titanium, the protein expression of OPG increased linearly with time, especially after 96 hours, while the protein expression of RANKL did not show significant changes with titanium processing. Given the increase in OPG expression after the initial cytotoxicity, changes in cytotoxicity with titanium may be attributable to the antagonistic effect of OPG on cytotoxicity."

5 2011

철 킬레이터에 의한 골모세포 분화유도에서 Heme Oxygenase-1의 관여

저자 함선도, 이선경, 국윤아, 김용일, 신경섭, 이소윤, 배원정, 이상임, 이영만, 강순일, 박재국, 류원국, 채종문, 이승훈

초록

"The present study aimed to verify the effects of DFO on PDL cells, with particular emphasis on focusing on osteoblastic differentiation. Its mechanisms related to heme oxygenase-1 (HO-1) pathway were also analyzed. DFO increased the expression of HO-1 and early osteoblastic differentiation markers, such as alkaline phosphatase (ALP) and bone sialoprotein (BSP). DFO upregulated heme oxygenase-1. Treatment with HO-1 siRNA blocked the DFO-stimulated osteoblastic differentiation and HO-1 expression. The NF-kB inhibitor pyrrolidine dithiocarbamate, phosphatidylinositol 3-kinase inhibitor Wortmannin, and p38 MAPK inhibitor U0126 blocked the effects of DFO on HO-1 expression and osteoblastic differentiation in PDL cells. Collectively, these data suggest that DFO promotes osteoblastic differentiation and induces the expression of defense protein HO-1 probably via PI3K, p38 MAPK, and NF-kB signalling pathways in PDL cells."

4 2011

포토프린을 이용한 광역학 치료와 시스플라틴 혹은 카보플라틴을 이용한 병용치료

저자 임현주, 오충훈

초록

"Photodynamic therapy(PDT) is recently developed as an effective treatment for malignant disease. Carboplatin, a less nephrotoxic analog of cisplatin, has been widely used for the treatment of multiple malignancies. In this study, we investigated the cytotoxic and apoptotic effect of combined modality of photofrin mediated PDT with cisplatin and carboplatin on KB cell human oral cancer cell line in vitro. The attached KB cells were incubated with cisplatin(0.04mg/ml) and carboplatin(0.02mg/ml) for 24h at 37℃ and followed by photosensitization with photofrin for 6h and laser irradiation with 630nm LED at an intensity of 2.0 J/cm2 for activating photofrin for 15min. Then MTT assay and SYTO 16 green & Propidium iodide (PI) double staining were used respectively to measure the cytotoxicity and nuclear morphology at 24h after PDT.This study demonstrates that the combined modality with carbopaltin resulted in enhanced apoptotic cell death as well as cytotoxic effect on KB cells in vitro, which suggests the feasibility of combined modality and the possibility of reducing the effective dosage of photofrin and carboplatin and lowering the side effects on normal cells."

3 2011

다이오드레이저 조사 시 임플란트 계면에서 신생골 형성에 관한 반사전자현미경적 연구

저자 최진호, 천재식, 이석준

초록

"Currently, dental implants are generally used for reconstruction of oromaxillofacial defects. Implants are widely used in dental and medical fields. The materials of implants are variable such as metals and ceramics. The materials of implants must be have not toxicity and biocompatibility to host and mechanical(physical) strength. Bones must be attached to titanium surface without any other tissues. many researcher's had studied for raising the osseointegration through various method which are including implants designs and materials. It was reported over 95% success ratio. many researcher's study the methods which are enhancing the speed of bone remodelling and osseointegration. Thermo dynamic therapy is one of the method to accelerlate the speed of bone remodelling and osseointegration. Thus it raise stability of implants. The purpose of this study was to evaluate the effect of diode laser irradiation for ossoeintegration in implant interface and between the implants threads. 24 New Zealand white rabbits which were about 3kg weight, used for experiment. 2 implant's were implanted every rabbit's tibia. 2 weeks, 4 weeks, 8 weeks after implantation, tissue sample were removed from sacrificed rabbit's tibia. 8 rabbit's were sacrificed every 2, 4, 8 weeks and undecalcified sample were made from tissue sample. We have investigated the undecalcified samples by back scattered electron microscope. We have analysied the length rate and area rate in implant interface and inside the threads. The results were as follows. 2 weeks, 4 weeks, 8 weeks experimental groups which were irradiated low level laser therapy showed rapid bone remodelling than control groups. It was suggested that Initial bone remodelling may be effected by LLLT because of implant bone contact ratio between 4th weeks and 8th weeks had no siginifant difference. Initial bone remodelling may be more influenced than later bone remodelling by LLLT because of new bone formation area ratio between implant threads had no significant differences during 4th to 8th weeks. According to above results, low level laser irradiation accerlate the new bone formation in implant interface and inside the implants threads at initial stage. there were many factors which are increasing the bone remodelling, because there were many differences between experimental and control groups. Low level laser irradiation were helpful for increasing the initial stage of bone remodelling because of above results."

2 2011

Emodin에 의한 Myeloid Cell Leukemia-1 단백질 억제를 통한 점액표피양암종세포주에서의 apoptosis 유도 기전

저자 최경희, 조남표, 조성대

초록

"Emodin is a bioactive compound isolated from the root and rhizomes of Rheum plamatum L. (polygenaceae), which is known as a traditional Chinese and Japanese medicine. In the present study, the effect of emodin on YD-15 mucoepidermoid carcinoma cells and its molecular mechanism were investigated. This study shows that emodin significantly inhibits the growth of YD-15 cells. Activation of caspase-3 and PARP is triggered by emodin and it increases sub-G1 population and the number of YD-15 cells with nuclear condensation and fragmentation. In addition, we found that emodin significantly decreases myeloid cell leukemia 1(MCL-1). These results suggest that MCl-1 is an important molecule for emodin-induced apoptosis. Taken together, emodin inhibits cell viability and induces apoptosis via down-regulation of MCL-1 and it can be a new potent anticancer drug candidate for the treatment of mucoepidermoid carcinoma."

1 2011

저산소증이 구강편평세포암종의 상피-간엽 전환에 미치는 영향

저자 김경아, 김지영, 유미현, 박봉수, 정진, 박혜련

초록

"Malignant tumor cells outgrow new blood vessel formation and tend to be in hypoxic state. Hypoxic cancer cells adapt to hypoxic conditions by transforming its characteristics. On the other hand, one of the most important features of cancer cells is that carcinoma cells loses its inherent epithelial phenotype and acquires mesenchymal characteristics, called as epithelial-mesenchymal transition(EMT). It has been already well known that EMT contributes to tumor invasion and metastasis.The present study investigated whether hypoxia play a major role in induction of phenotypic changes of oral squamous cell carcinoma(OSCC). Furthermore, the mechanism of EMT in oral squamous cell carcinoma cells by hypoxia has been clarified.To mimic hypoxic condition, cobalt chloride and desferoxamine, well-known hypoxic mimetic agents, were used. This study shows that hypoxia suppresses the expression of E-cadherin(epithelial marker) and increases vimentin and N-cadherin(mesenchymal markers) in OSCC. In addition, α5 integrin protein, which is a receptor for fibronectin and an important molecule for tumor invasion, is prominently induced by hypoxia."

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